Friday, September 03, 2010
 
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Oxidative Stress in High Producing Dairy Cows
Larry R. Prewitt, Ph.D.
President and Owner of Prewitt Consulting Firm
Dairy producers readily recognize the effect of metabolic disorders on herd health and profitability. Milk fever, retained placenta, udder edema and reproductive efficiency are problems associated with calving and the onset of lactation. If not managed appropriately, profitability is severely reduced. Researchers have suggested that oxidative stress may contribute to the occurrence of metabolic disorders. To help dairy producers better understand oxidative stress, this article will define oxidative stress, explain why and how oxidative stress affects high producing cows and, in conclusion, provide a few solutions to minimize oxidative stress.
   
Definition of Oxidative Stress
In simple terms, oxidative stress can be defined as a condition where an imbalance occurs between the production of free radicals (compounds that have given up electrons during metabolism and are therefore highly reactive) and the body’s antioxidant mechanisms. Antioxidants are compounds that function as scavengers by donating electrons to free radicals that make them stable and safe in the body.

To combat the oxidation of dietary ingredients and production of free radicals, the body maintains a variety of antioxidant defense mechanisms. Without an antioxidant defense system working to neutralize free radicals, they will continue to accumulate, causing toxicity in the animal. This can result in cellular damage and inefficiencies within the cell, eventually leading to death of the cell. Cellular death equates to an increase in the maintenance cost to the cow, which means less energy available to milk production, growth, immune function and reproduction.
   
Impact of Oxidative Stress in High Producing Cows
High-producing cows are prone to oxidative stress in the same manner they are prone to having metabolic disorders. It is well recognized that the onset of events such as calving, rapid elevation of milk production, extreme heat conditions and unbalanced rations are factors that lead to the occurrence of metabolic disorders. These same conditions affect the level of oxidative stress.

Many dairy producers utilize special dry cow programs to give the cow a rest during the final period of calf growth. It is a time for properly conditioning the cow and replenishing body reserves for mobilization during the lactation. The use of effective forage particles appropriately balanced with quality concentrates, bioavailable trace minerals and vitamins helps maintain a healthy digestive system and proper body condition for the cow.

These management practices have been demonstrated to reduce the occurrence of metabolic disorders and also oxidative stress. They help the cow improve feed intake and reach peak production quicker. The net result is higher production, better breeding efficiency, shorter calving intervals and greater profitability.

Oxidative stress has been hypothesized to be related to metabolic disorders and antioxidant deficiency in dairy cows. Symptoms of lactating cows with oxidative stress include retained placenta, udder edema and mastitis. Other visible symptoms include delayed first estrus, delayed first breeding, repeat breeding resulting from failure to conceive or from early embryonic death. Symptoms of oxidative stress result in increased calving intervals, lowered production, and ultimate culling of cows. All of these symptoms are costly and reduce profitability for dairy producers. It has been estimated that more than 50% of health costs occur between calving and the first 30 days in milk. This is the same period that the majority of metabolic disorders and oxidative stress occur in high-producing cows.

It has been demonstrated that the level of alpha tocopherol is lowest during the periparturient period. Supplying antioxidants during this period could help protect the animal against lipid peroxidation, which is an indicator of oxidative stress.

Dietary ingredients have an effect upon oxidative stress. High quantities of fat are routinely supplied to high producing cows to increase the energy density of the diet. The effect of the fat on the animal is dependent on whether or not the fat has been stabilized with an antioxidant. Stabilized lipid supplements are less likely to contribute to elevating peroxides coming from the diet, therefore reducing the overall oxidative load on the animal. Peroxides cause damage to cellular membranes, internal cellular components and proteins. Dairy producers must consider all dietary sources of fat supplied to the cow. More than 50% of dietary fat can come from sources other than supplemental fat. Typical sources may include cottonseed (19% fat), distillers grains (5-15% fat), soybean meal (7-20% fat), and fish meal (10% fat). Thus, steps may be taken to include antioxidants in the diet to stabilize these ingredients and help manage oxidative stress prior to feeding.
   
Description of Antioxidant Defense Mechanism
Among the antioxidant defense mechanisms prevalent in the cow are enzymes that routinely serve as scavengers of radical compounds. Two enzymes-superoxide dismutase

(SOD) and glutathione peroxidase (GSH-Px) – serve as the first line of intracellular defense against peroxidation.

Activity of antioxidant enzymes is very rapid and utilizes trace elements as catalysts. For example, SOD in the presence of copper, manganese and zinc converts superoxide to hydrogen peroxide. Selenium is another trace element used in some reactions. Hydrogen peroxide has the adverse effect of attacking the lipid membrane of cell walls, which ultimately results in cell death. Hydrogen peroxide is neutralized by GSH-Px, which converts hydrogen peroxide to safe compounds for the body. Selenium is a key mineral catalyst for glutathione peroxidase.

As a result of these two antioxidant enzymes, SOD and GSHPx, free radicals are neutralized resulting in no cell or tissue damage. If free radicals such as superoxide and hydrogen peroxide were left unchecked and continued to accumulate, the compounds would continue attacking the lipid cell membrane, ultimately causing cell death and health problems in the cow.

Other antioxidants used by the antioxidant defense mechanism are non-enzymatic protein antioxidants located in the extracellular fluid. These antioxidants are composed of total thiol groups representing the sulfhdryl groups of albumin, L-cysteine and homocysteine. Protein sulfhydryl groups are considered a significant element of the extracellular antioxidant defense system against oxidative stress.

The third group of the antioxidant defense system consists of nonenzymatic low-molecular weight antioxidants. These compounds consist of water-soluble antioxidants (ascorbic acid, glutathione and uric acid) and lipid-soluble antioxidants (vitamin E). These antioxidants are found in plasma, extracellular fluids, intracellular fluids, lipoproteins and membranes. An example of the performance of these antioxidants is with mastitis. In clinical mastitis, it has been demonstrated that as lipid peroxides increase there is a subsequent drop in ascorbic acid over that of healthy animals.

   
Conclusions
High-producing cows are prone to oxidative stress. Conditions that promote the production and accumulation of free radicals in excess of the neutralizing capacity of antioxidant defense mechanisms result in oxidative stress. High-producing cows are more susceptible to oxidative stress during the periparturient and early stages of lactation. The onset of calving, rapid acceleration of milk production, mobilization of body reserves, high ambient temperature and disease conditions are all disorders that contribute to oxidative stress. Follow good feeding practices known to reduce oxidative stress such as quality feeding programs that are appropriately balanced in nutrients, antioxidants, bioavailable trace elements and vitamins.
   
Practical Solutions for Overcoming Oxidative Stress
High-producing cows are prone to oxidative stress. To help control problems associated with oxidative stress, dairy producers can:
  • Supply antioxidants in the diet to stabilize fat and help reduce the accumulation of free radicals that cause oxidative stress
  • Provide a balanced ration including antioxidants
  • Include an adequate balance of bioavailable trace minerals and vitamins known to be key for certain antioxidant relevant enzymes
  • Provide a sufficient dry period to give the cow rest and time for calf growth
  • Supply a well-balanced ration late in lactation and through the dry period to avoid over conditioning cows
  • Provide adequate housing to keep cows clean and comfortable to reduce stress
  • Keep cows cool during high ambient temperature to reduce oxidative stress
  • Provide routine health evaluations by a veterinarian to keep your herd free of disease, which helps minimize oxidative stress.

   
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